hTROP2

Fig1. Expression characterization of hTROP2 in hTrop2 KI mice. (In collaboration with the third party)

Fig2. Detection of hTROP2 expression in cervix and kidney in heterozygous hTROP2 knockin mice by IHC.

Abbr. HE, heterozygous;  WT, wild type.

Fig.3 Blood routine test of HE hTROP2 knockin mice.

Fig.4 Blood Biochemistry of of HE hTROP2 knockin mice. 

Fig.5 Basic pathological analysis of hTROP2 mice. Tissues including the small intestine, liver, eyeball, and skin were collected for hematoxylin–eosin (HE) staining.

Fig.5 Detection of TROP2 expression in skin by RT-PCR. Skin RNA was extracted from 11-week-old female wild-type C57BL/6 (WT)(n=3) and homozygous hTROP2 knockin mice (HO) (n=3), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mRNA primers. 

Abbr. M, marker; HO, homozygous; WT, wild type.

Fig.6 Detection of TROP2 expression in hTROP2 mice by WB. The lung, kidney, bladder, uterus and stomach tissue lysates were collected from 10-week-old female wild-type C57BL/6 mice and 10-week-old female homozygous hTROP2 mice, and then analyzed by western blot with anti-TROP2 antibody. 

Abbr. HO, homozygous; WT, wild type; PC, positive control, HCT116 cells.

Fig 7.Frequency of leukocyte and myeloid subpopulations in the spleen by flow cytometry. Spleen was collected from WT C57BL/6 mice and HO hTROP2 mice (10-week-old, female, n=3). Flow cytometry analysis of frequencies of (A) lymphocyte and (B) myeloid subpopulations in the spleen. Bar indicated expressed as mean ± SEM.

Abbr.  HO, homozygous; WT, wild type.

Fig 8.Frequency of leukocyte and myeloid subpopulations in the blood by flow cytometry. Blood was collected from WT C57BL/6 mice and HO hTROP2 mice (10-week-old, female, n=3). Flow cytometry analysis of frequencies of (A) lymphocyte and (B) myeloid subpopulations in the blood. Bar indicated expressed as mean ± SEM.

Abbr.  HO, homozygous; WT, wild type.

Fig 9.Frequency of leukocyte and myeloid subpopulations in the lymph node by flow cytometry. Lymph node was collected from WT C57BL/6 mice and HO hTROP2 mice (10-week-old, female, n=3). Flow cytometry analysis of frequencies of lymphocyte in the lymph node. Bar indicated expressed as mean ± SEM.

Abbr.  HO, homozygous; WT, wild type.