hCPT1B

Nomenclature

C57BL/6JSmo-Cpt1btm1(hCPT1B)Smoc

Cat. NO.

NM-HU-234651

Strain State

Embryo Cryopreservation

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Gene Summary

Gene Symbol
Cpt1b

Model Description

The endogenous mouse Cpt1b gene was replaced by human CPT1B gene.

Validation Data

image.png

Fig. 1 Detection of CPT1B expression in heart by RT-PCR (female mice in both groups). 

Wild type: only one band at 378 bp with primers F1/R1(mCpt1b);

Homozygous:  only one band at 634 bp with primers F2/R2(hCPT1B).

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

image.png

Fig.2 Detection of CPT1B expression in hCPT1B knockin mice by WB. 

Various tissue lysates were collected from wild-type C57BL/6J mice (WT, female, 8-10 weeks old) and homozygous hCPT1B mice (HO, female, 11 weeks old), and then analyzed by western blot with anti-CPT1B antibody (CST, 41803T). 20 μg total proteins were loaded for western blotting analysis.


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