HLA-DPA1/HLA-DPB1

Fig.1 Detection of HLA-DPA1/HLA-DPB1 expression in large intestine and spleen by RT-PCR. 

Wild type: one band at 278 bp with primers F4/R4(mH2-Aa) and one band at 123 bp with primers F/R(mGapdh);

Heterozygous: one band at 278 bp with primers F4/R4(mH2-Aa) and one band at 107 bp with primers F1/R1(hHLA-DPA1) and one band at 123 bp with primers F/R(mGapdh).

Abbr. M, DNA marker; HE, heterozygous; WT, wild type.

Fig.2 Detection of HLA-DPA1/HLA-DPB1 expression in large intestine and spleen by RT-PCR. 

Wild type: one band at 299 bp with primers F3/R3(mH2-Ab1) and one band at 123 bp with primers F/R(mGapdh);

Heterozygous: one band at 299 bp with primers F3/R3(mH2-Ab1), one band at 113 bp with primers F2/R2(hHLA-DPB1) and one band at 123 bp with primers F/R(mGapdh).

Abbr. M, DNA marker; HE, heterozygous; WT, wild type.

Fig.3 Detection of MHC-Ⅱ expression on B cells and DCs in Peripheral blood in 13-week-old WT C57BL/6 and HE HLA-DPA1/HLA-DPB1 mice.

Fig.4 Detection of MHC-Ⅱ expression on B cells and DCs in Spleen in 13-week-old WT C57BL/6 and HE HLA-DPA1/HLA-DPB1 mice.

Fig.5 Detection of MHC-II expression on B cells in blood  of WT C57BL/6 mice and HO HLA-DPA1/HLA-DPB1 mice by FACS. Leukocytes were collected from wild-type C57BL/6 mice (male, 6 weeks old) and homozygous HLA-DPA1/HLA-DPB1 humanized mice (male, 6 weeks old), and analyzed by flow cytometry with anti-mMHC-II and anti-hMHC-II. hMHC-II was exclusively detectable in HLA-DPA1/HLA-DPB1 humanized mice but not in wild-type mice. hMHC-II was exclusively detectable on B cells in HLA-DPA1/HLA-DPB1 humanized mice, whereas mMHC-II was exclusively detectable on B cells in wild-type C57BL/6 mice.

Abbr. HO, homozygous; WT, wild type.

Fig.6 Detection of MHC-II expression on B cells and DCs in spleen of WT C57BL/6 mice and HO HLA-DPA1/HLA-DPB1 mice by FACS. Leukocytes were collected from wild-type C57BL/6 mice (male, 6 weeks old) and homozygous HLA-DPA1/HLA-DPB1 humanized mice (male, 6 weeks old), and analyzed by flow cytometry with anti-mMHC-II and anti-hMHC-II. hMHC-II was exclusively detectable in HLA-DPA1/HLA-DPB1 humanized mice but not in wild-type mice. hMHC-II was exclusively detectable on B cells and DCs in HLA-DPA1/HLA-DPB1 humanized mice, whereas mMHC-II was exclusively detectable on B cells and DCs in wild-type C57BL/6 mice.

Abbr. HO, homozygous; WT, wild type.

Fig 7. Frequency of leukocyte subpopulations in the blood by flow cytometry

Blood was collected from WT C57BL/6 mice and HO HLA-DPA1/HLA-DPB1 mice (7-week-old, n=3). Flow cytometry analysis of frequencies of (A) female and (B) male subpopulations in the blood. Bar indicated expressed as mean ± SEM.

Abbr.  HO, homozygous; WT, wild type.

Fig 8. Frequency of myeloid subpopulations in the blood by flow cytometry

Blood was collected from WT C57BL/6 mice and HO HLA-DPA1/HLA-DPB1 mice (7-week-old, n=3). Flow cytometry analysis of frequencies of (A) female and (B) male subpopulations in the blood. Bar indicated expressed as mean ± SEM.

Abbr.  HO, homozygous; WT, wild type.

Fig 9. Frequency of leukocyte subpopulations in the spleen by flow cytometry

Spleen was collected from WT C57BL/6 mice and HO HLA-DPA1/HLA-DPB1 mice (7-week-old, n=3). Flow cytometry analysis of frequencies of (A) female and (B) male subpopulations in the spleen. Bar indicated expressed as mean ± SEM.

Abbr.  HO, homozygous; WT, wild type.

Fig 10. Frequency of myeloid subpopulations in the spleen by flow cytometry

Spleen was collected from WT C57BL/6 mice and HO HLA-DPA1/HLA-DPB1 mice (7-week-old, n=3). Flow cytometry analysis of frequencies of (A) female and (B) male subpopulations in the spleen. Bar indicated expressed as mean ± SEM.

Abbr.  HO, homozygous; WT, wild type.