hCD3EDG/hDLL3

Nomenclature

C57BL/6JSmo-Cd3etm1(hCD3E)Cd3dtm1(hCD3D)Cd3gtm1(hCD3G)Dll3tm1(hDLL3)Smoc

Cat. NO.

NM-XA-240036

Strain State

Repository Live

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Gene Summary

Gene Symbol
Dll3

Synonyms

pu; pudgy

NCBI ID

13389

MGI ID

1096877

Ensembl ID

ENSMUSG00000003436

Pubmed

Dll3

Human Ortholog

DLL3

Model Description

The hDLL3(NM-HU-200231) was crossed with hCD3EDG(NM-HU-220120) to generate hCD3EDG/hDLL3 mice.

Validation Data

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Fig.1 Detection of humanized DLL3 expression in male hCD3EDG/hDLL3 mice by RT-PCR. 

Homozygous:  one band at 282 bp with primers F2/R2(hDLL3). 

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

image.png

Fig.2 Detection of humanized DLL3 expression in female hCD3EDG/hDLL3 mice by RT-PCR. 

Homozygous: one band at 282 bp with primers F2/R2(hDLL3). 

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

image.png

Fig.3 Detection of mouse Dll3 expression in male hCD3EDG/hDLL3 mice by RT-PCR. 

Wild type: one band at 172 bp with primers F1/R1(mDll3). 

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

image.png

Fig.4 Detection of mouse Dll3 expression in female hCD3EDG/hDLL3 mice by RT-PCR.

Wild type: one band at 172 bp with primers F1/R1(mDll3). 

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

image.png

Fig.5 Detection of mouse Gapdh expression in male hCD3EDG/hDLL3 mice by RT-PCR. 

Wild type and homozygous: one band at 123 bp with primers F/R(mGapdh). 

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

image.png

Fig.6 Detection of mouse Gapdh expression in female hCD3EDG/hDLL3 mice by RT-PCR. 

Wild type and homozygous: one band at 123 bp with primers F/R(mGapdh). 

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

image.png

Fig.7 Detection of human DLL3 expression in 5 embryos (E10.5~E14.5), whose parents are both HO hCD3EDG/hDLL3 mice, by WB. 

Abbr. HO, homozygous.

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Fig.8 Detection of human DLL3 expression in 7-14-week-old hCD3EDG/hDLL3 knockin mice by WB. 

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Fig.9 Detection of (A) body weight, (B) the weights of different tissues and (C) organ index in HO hCD3EDG/hDLL3 mice.

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Fig.10 Detection of mIgG(A)  and mIgM(B) expression in serum by ELISA. 

Abbr. HO, homozygous; WT, wild type.

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Fig.11 Detection of immune phenotype in (A) peripheral blood and (B) spleen by FACS.  

Abbr. HO, homozygous; WT, wild type.


Table 1. Blood routine examination in hCD3EDG/hDLL3 dKI mice (n=3 /group).

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