Muc2-2A-CreERT2-IRES-tdTomato

Nomenclature

C57BL/6JSmo-Muc2em1(2A-CreERT2-tdTomato-Wpre-pA)Smoc

Cat. NO.

NM-KI-200301

Strain State

Repository Live

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Gene Summary

Gene Symbol
Muc2

Synonyms

MCM; wnn; 2010015E03Rik

Chromosome

7

NCBI ID

17831

MGI ID

1339364

Ensembl ID

ENSMUSG00000025515

Pubmed

Muc2

Model Description

2A-CreERT2-tdTomato-Wpre-pA expression cassette was knocked into the Muc2 gene stop codon site.

Validation Data

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Fig 1. Active tdTomato expression was observed in the large intestine of Muc2CreERT2-IRES-tdTomato/+  mouse. WT, wild type as control.

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Fig 2. Active tdTomato expression was observed  in the small intestine of Muc2CreERT2-IRES-tdTomato/+ mouse. WT, wild type as control.

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Fig 3. No obvious tdTomato signal was observed in the stomach of Muc2CreERT2-IRES-tdTomato/+ mouse. WT, wild type as control.

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Fig 4. Active tdTomato signal was observed in the colon of Muc2CreERT2-IRES-tdTomato/+ mouse. WT, wild type as control.

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Fig 5. Active tdTomato signal was observed in the cecum of Muc2CreERT2-IRES-tdTomato/+ mouse. WT, wild type as control.

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Fig 6. TdTomato signal was observed in the small intestine of Muc2CreERT2-IRES-tdTomato/+ mouse.WT, wild type as control.


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Fig 7. No obvious tdTomato signal was observed in the stomach of Muc2CreERT2-IRES-tdTomato/+ mouse. WT, wild type as control.

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Fig8. CreERT2 mediated recombination in colon of Muc2CreERT2-IRES-tdTomato/+; Rosa26zsGreen/+ mouse. 

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Fig9. Detection of tdTomato(red) and zsGreen(green) in various tissues of  Muc2CreERT2-IRES-tdTomato/+; Rosa26zsGreen/+ mouse.  Following tamoxifen induction, zsGreen expression was observed in the stomach, large intestine, small intestine, and thymus.  that tdTomato was expressed in the stomach, large intestine, and small intestine.(57-days old, male) (For more detailed information please contact our technical advisor.) 

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