H11-CAG-LSL-ZsGreen-CAG-RSR-tdTomato

Nomenclature

C57BL/6JSmo-Igs2em1(CAG-LSL-ZsGreen-wpre-pA-CAG-RSR-tdTomato-wpre-pA)Smoc

Cat. NO.

NM-KI-200319

Strain State

Repository Live

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Model Description

A CAG-LSL-ZsGreen-wpre-pA-CAG-RSR-tdTomato-wpre-pA expression cassette was knocked into the H11 locus.Tissue sections showed tissues with leaky ZsGreen expression: brain, spinal cord, lungs, stomach, salivary glands, kidney, large intestine, reproductive system, skin, pancreas, and thymus; tissues with leaky tdTomato expression: liver, spleen, and reproductive system.

Validation Data

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Fig1. Detection of zsGreen and tdTomato expression in heart and lung of H11CAG-LSL-ZsGreen-CAG-RSR-tdTomato/+ mice.

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Fig2. Detection of zsGreen and tdTomato expression in brown fat, epididymis and testis of H11CAG-LSL-ZsGreen-CAG-RSR-tdTomato/+ mice.

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Fig3. Detection of zsGreen and tdTomato expression in pancreas of H11CAG-LSL-ZsGreen-CAG-RSR-tdTomato/+ mice.

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Fig4. Detection of zsGreen and tdTomato expression in brain of H11CAG-LSL-ZsGreen-CAG-RSR-tdTomato/+ mice.

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Fig5. Detection of zsGreen and tdTomato expression in liver of H11CAG-LSL-ZsGreen-CAG-RSR-tdTomato/+ mice.

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Fig6. Detection of zsGreen and tdTomato expression in spleen of H11CAG-LSL-ZsGreen-CAG-RSR-tdTomato/+ mice.


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Fig7. Detection of zsGreen and tdTomato expression in thymus of H11CAG-LSL-ZsGreen-CAG-RSR-tdTomato/+ mice.

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Fig8. Detection of zsGreen and tdTomato expression in salivary gland of H11CAG-LSL-ZsGreen-CAG-RSR-tdTomato/+ mice.

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Fig9. Detection of zsGreen and tdTomato expression in small intestine of H11CAG-LSL-ZsGreen-CAG-RSR-tdTomato/+ mice.

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Fig10. Detection of zsGreen and tdTomato expression in colon of H11CAG-LSL-ZsGreen-CAG-RSR-tdTomato/+ mice.

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Fig11. Detection of zsGreen and tdTomato expression in kidney of H11CAG-LSL-ZsGreen-CAG-RSR-tdTomato/+ mice.

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Fig12. In the pancreas of the H11CAG-LSL-ZsGreen-CAG-RSR-tdTomato/+ genetically modified mouse, leaky ZsGreen fluorescence expression was observed, whereas no significant leaky tdTomato fluorescence expression was detected.

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Fig13. Detection of tdTomato(red) and zsGreen(green) in various tissues of H11CAG-LSL-ZsGreen-CAG-RSR-tdTomato/+ mouse. In H11CAG-LSL-ZsGreen-CAG-RSR-tdTomato positive mice, tissues with leaky ZsGreen expression: brain, spinal cord, lungs, stomach, salivary glands, kidney, large intestine, reproductive system, skin, pancreas, and thymus.

Tissues with leaky tdTomato expression: liver, spleen, and reproductive system.

Mouse age: 37Day  ; Male.

Publications 1piece

1. A novel lineage of osteoprogenitor cells with dual epithelial and mesenchymal properties govern maxillofacial bone homeostasis and regeneration after MSFL
Published year：2022-07-12 References：Cell Research

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