R26-CreERT2

Nomenclature

C57BL/6JSmo-Gt(ROSA)26Sorem1(SA-FRT-2xpolyA-CAG-FRT-CreERT2-Rox-WPRE-Rox-polyA)Smoc

Cat. NO.

NM-KI-200041

Strain State

Repository Live

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Gene Summary

Gene Symbol
Gt(ROSA)26Sor

Synonyms

R26，ROSA26，AV258896，Gtrgeo26，Gtrosa26，Thumpd3as1

NCBI ID

14910

MGI ID

104735

Ensembl ID

ENSMUSG00000086429

Pubmed

Gt(ROSA)26Sor

Model Description

These mice harbor a SA-FRT-2xpolyA-CAG-FRT-CreERT2-Rox-WPRE-Rox-polyA cassette in the Rosa26 locus generated by homologous recombination. When crossed with a strain carrying a gene flanked by loxP sites, the flanked gene will be removed in multiple tissues after tamoxifen treatment.
Research Application：Cre recombinase tool

Validation Data

creERT2-1.png

Fig1. Detection of tdTomato(red) in various tissues of R26CreERT2/+; Rosa26tdTomato/+ mice by fluorescence assay. Expression tissues include: brain, liver, stomach, thymus, pancreas, colon, heart, kidney and spleen. (For more detailed information please contact our technical advisor.) 

creERT2-2.png

Fig2. CreERT2-mediated recombination in peripheral blood of R26CreERT2/+; Rosa26tdTomato/+ mice.

a3dfaae7-3244-496f-b70c-1593988c45a0.png

Fig3. CreERT2-mediated recombination in pancreas of  R26CreERT2/+; Rosa26tdTomato/+ mice.

9feac681-ad5d-47a6-a734-71ecb15f171a.png

Fig2. Detection of tdTomato(red) in various tissues of R26CreERT2/+;Rosa26 tdTomato/+ mouse. 

Tissues with cellular expression after tamoxifen induction include: brain, eye, heart, lung, liver, stomach, adrenal gland, pituitary gland, salivary gland, brown adipose tissue, kidney, large intestine, small intestine, spleen, reproductive system, muscle, skin, pancreas, thymus, dorsal root ganglion, and sciatic nerve.

Cellular leaky expression is also present in the brain, eye, heart, lung, liver, stomach, adrenal gland, pituitary gland, salivary gland, brown adipose tissue, kidney, large intestine, small intestine, spleen, reproductive system, muscle, skin, pancreas, thymus, dorsal root ganglion, and sciatic nerve.

Mouse age: 62 days; Female. (For more detailed information please contact our technical advisor.) 


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