Glp1r-KO

Fig.1 Detection of Glp1r expression in lung by RT-PCR. 

Wild type: one band at 117 bp with primers F1/R1 (mGlp1r) and one band at 164 bp with primers F2/R2 (mGlp1r). 

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

Fig.2 Detection of Glp1r expression in heart, lung, kidney, brain and pancreas by qPCR. Mouse Glp1r mRNA was detectable only in wild-type mice but not in homozygous Glp1r-KO mice. Heart, lung, kidney, brain and pancreas RNA was extracted from 8-week-old male wild-type C57BL/6 (WT) (n=3) and homozygous Glp1r-KO mice (HO) (n=3), then cDNA libraries were synthesized by reverse transcription, followed by qPCR with mouse Glp1r and mouse Gapdh primers. Relative expression represents the mouse Glp1r mRNA level relative to its average expression of kidney in wild-type mice .

Abbr. M, marker; HO, homozygous; WT, wild type.

Fig.3 Immunohistochemical analysis of mouse Glp1r expression across various tissues in Glp1r-KO mice. IHC staining revealed GLP1R-positive signals in the brain, lung, pancreas, and kidney of WT C57BL/6 mice, but not in Glp1r-KO mice. No GLP1R immunoreactivity was detected in the heart of either genotype. Brains, lungs, pancreas, kidneys, and hearts were collected from Glp1r-KO and WT C57BL/6 mice (n=3 males, 8 weeks old), fixed in 4% paraformaldehyde, and subjected to immunohistochemical (IHC) staining using a mouse-specific GLP1R antibody). Each image represents one mouse. Red arrows indicate areas of positive staining. Scale bar, 200 μm.

Abbr. M, marker; HO, homozygous; WT, wild type.