Gba-L462P
Nomenclature
C57BL/6JSmo-Gbaem(L462P)Smoc
Cat. NO.
NM-KI-225100
Strain State
Repository Live
Model Description
Validation Data
Fig.1 Detection of Gba expression in liver, spleen and brain by RT-PCR. Mouse Gba mRNA (432 bp) and mouse Gapdh mRNA (123 bp) was detectable both in wild-type C57BL/6 mice and heterozygous Gba-L462P mice. Liver, spleen and brain RNA was extracted from 32-week-old male wild-type C57BL/6 (WT)(n=2) and heterozygous Gba-L462P mice (HE) (n=2), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mRNA primers.
Abbr. M, marker; HE, heterozygous; WT, wild type.
Fig.2 mRNA sequencing of Gba-L462P knockin mice. Sequencing data of RT-PCR products confirmed the presence of target mutations in Gba-L462P mice.
Abbr. HE, heterozygous; WT, wild type.
Fig.3 Detection of GC activity expression in liver homogenate of WT C57BL/6 mice and HE Gba-L462P mice by ELISA. GC activity was detectable in liver homogenate from both WT C57BL/6 and heterozygote Gba-L462P knockin mice. Liver tissues lysates were collected from wild-type C57BL/6 mice (n=3, male, 34 weeks old) and HE Gba-L462P mice (n=3, male, 34 weeks old), and analyzed by ELISA with Glucosylceramidase Activity ELISA kit.
Abbr. HE, heterozygote; WT, wild type; GC, Glucosylceramidase.
Fig.4 Detection of GC activity expression in spleen homogenate of WT C57BL/6 mice and HE Gba-L462P mice by ELISA. GC activity was detectable in spleen homogenate from both WT C57BL/6 and heterozygote Gba-L462P knockin mice.Spleen tissues lysates were collected from wild-type C57BL/6 mice (n=3, male, 34 weeks old) and HE Gba-L462P mice (n=3, male, 34 weeks old), and analyzed by ELISA with Glucosylceramidase Activity ELISA kit.
Abbr. HE, heterozygote; WT, wild type; GC, Glucosylceramidase.
Fig.5 Detection of GC activity expression in brain homogenate of WT C57BL/6 mice and HE Gba-L462P mice by ELISA. GC activity was detectable in brain homogenate from both WT C57BL/6 and heterozygote Gba-L462P knockin mice. Brain tissues lysates were collected from wild-type C57BL/6 mice (n=3, male, 34 weeks old) and HE Gba-L462P mice (n=3, male, 34 weeks old), and analyzed by ELISA with Glucosylceramidase Activity ELISA kit.
Abbr. HE, heterozygote; WT, wild type; GC, Glucosylceramidase.
Fig. 6 Detection of α-Synuclein expression in the substantia nigra of Gba-L462P mice by IHC. α-Synuclein immunoreactivity was detectable in the substantia nigra of both WT C57BL/6 and heterozygous Gba-L462P mice. Representative images of α-synuclein staining in the substantia nigra of WT C57BL/6 mice (left) and heterozygous Gba-L462P mice (right) (n = 2 males).
Abbr. HE, heterozygous; WT, wild type.
You may also like
Shanghai Model Organisms Center Inc has licensed CRISPR-Cas9 technology from Broad Institute
On Dec 16, 2018, Broad Institute and Shanghai Model Organisms Center Inc (SMOC) has entered into a non-exclusive license agreement under which Broad has granted SMOC worldwide rights to commercialize a service platform for genetically modified mouse models under Broad's intellectual property.
Learn moreCustomizing Mouse Models: Delivering in 100 Days to Speed Up Your Research!
At GenoBioTX, we understand that the lengthy wait times for gene-modified mouse models can hinder your research progress. Traditional methods often require 6-9 months, leading to delays and increased costs. That’s why we’re thrilled to introduce our innovative service designed to streamline this process and deliver results faster.
Learn moreMilestones
Our vision is to provide researchers all over the world with comprehensive, convenient and professional animal model services to facilitate a simplified and highly-efficient approach towards uncovering the mysteries of life.
Learn more
