Clec4f-IRES-Cre-2A-tdTomato

Nomenclature

C57BL/6JSmo-Clec4fem1(IRES-iCre-2A-tdTomato)Smoc

Cat. NO.

NM-KI-200069

Strain State

Repository Live

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Gene Summary

Gene Symbol
Clec4f

Model Description

A IRES-iCre-2A-tdTomato expression cassette was knocked into the Clec4f gene stop codon site.
Research Application:Cre recombinase tool,Kupffer cell

Validation Data

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Fig1. Co-localization of tdTomato-positive cells with Clec4f was observed in the livers of Clec4f IRES-Cre-2A-tdTomato/+;Rosa26 tdTomato/+ mice.

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Fig2. No colocalization was observed between tdTomato-positive cells and anti-Clec4f staining in the spleens of Clec4f IRES-Cre-2A-tdTomato/+; Rosa26 tdTomato/+ mice.

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Fig.3 Detection of tdTomato expression in spleen of WT C57BL/6 mice and Clec4f-IRES-Cre-2A-tdTomato mice by FACS. tdTomato expression was not detected on CD45+ cells in Clec4f-IRES-Cre-2A-tdTomato mice. Leukocytes were collected from wild-type mice (female,9 weeks old) and Clec4f-IRES-Cre-2A-tdTomato mice (male,10 weeks old) .

Abbr. HO,  homozygous;WT, wild type.

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Fig.4 Detection of tdTomato expression in liver of WT C57BL/6 mice and Clec4f-IRES-Cre-2A-tdTomato mice by FACS. tdTomato expression was detected on CD11b low F4/80+ cells in Clec4f-IRES-Cre-2A-tdTomato mice. Leukocytes were collected from wild-type mice (female,9 weeks old) and Clec4f-IRES-Cre-2A-tdTomato mice (male,10 weeks old) .

Abbr. HO,  homozygous;WT, wild type.



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