Cd79a-2A-Cre

Nomenclature

C57BL/6JSmo-Cd79aem(2A-Cre)Smoc

Cat. NO.

NM-KI-220089

Strain State

Repository Live

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Gene Summary

Gene Symbol
CD79a

Model Description

2A-Cre expression cassette was knocked into the Cd79a gene stop codon site.
Research Application:Cre mouse

Validation Data

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Fig1. Cre mediated recombination in kidney of Cd79aCre/+; Rosa26tdTomato/+ mouse. 

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Fig2. Detection of tdTomato(red) in various tissues of Cd79aCre/+; Rosa26tdTomato/+ mouse. 

Tissues expressing tdTomato: stomach, salivary gland, adrenal gland, kidney, pancreas, large intestine, small intestine, spleen, genitalia, thymus, pituitary gland. Low expression in tissues: brain, lung, skin. Non-expressing tissues: heart, eye, liver, brown fat, spinal cord, muscle.  (9.6-weeks old, female) (For more detailed information please contact our technical advisor.) 

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Fig3. Detection of tdTomato expression in CD19+CD79a+ cells of spleen and peripheral blood by FACS.

Leukocytes were collected from HE Cd79a-2A-Cre mice (male, 16 weeks old) and HE/HE R26-CAG-LSL-tdTomato/Cd79a-2A-Cre mice (male, 4 weeks old), and analyzed by flow cytometry with anti-mCD79a. 

Abbr. HE, Heterozygote; WT, wild type.

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Fig4. Detection of tdTomato expression in CD19- cells of spleen and peripheral blood by FACS.

Leukocytes were collected from HE Cd79a-2A-Cre mice (male, 16 weeks old) and HE/HE R26-CAG-LSL-tdTomato/Cd79a-2A-Cre mice (male, 4 weeks old), and analyzed by flow cytometry with anti-mCD79a. 

Abbr. HE, Heterozygote; WT, wild type.


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