hRAMP1

Fig.1 Detection of RAMP1 expression in skin and brain by RT-PCR. Mouse Ramp1 mRNA (155 bp) was detectable only in wild-type C57BL/6 mice. Human RAMP1 mRNA (142 bp) was detectable only in homozygous hRAMP1 knockin mice but not in wild-type mice. 

Skin and brain RNA was extracted from 8-week-old female wild-type C57BL/6 (WT)(n=2) and homozygous hRAMP1 knockin mice (HO) (n=2), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mRNA primers. 

Abbr. M, marker; HO, homozygous; WT, wild type.

Fig.2 Detection of RAMP1 expression in hRAMP1 mice by WB. RAMP1 was detectable in brain and liver from both WT C57BL/6 and HO hRAMP1 mice, as the antibody was cross-reactived between human and mouse RAMP1. The brain and liver tissue lysates were collected from 8-week-old female wild-type C57BL/6 mice and 8-week-old female homozygous hRAMP1 mice, and then analyzed by western blot with anti-RAMP1 antibody. 

Abbr. HO, homozygous; WT, wild type; PC, positive control, MCF-7 cells.