hTP53-R273H

Fig.1 Detection of TP53 expression in spleen and thymus by RT-PCR. Spleen and thymus RNA was extracted from 6-week-old male wild-type C57BL/6 (WT)(n=1) and homozygous hTP53-R273H knockin mice (HO) (n=2), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mRNA primers. 

Abbr. M, marker; HO, homozygous; WT, wild type.

Fig.2 mRNA sequencing of hTP53-R273H knockin mice. 

Abbr. HO, homozygous; WT, wild type.

Fig.3 Detection of P53 expression in hTP53-R273H mice by WB. 

The spleen tissue lysates were collected from 6-week-old male wild-type C57BL/6 mice and 6-week-old male homozygous hTP53-R273H mice, and then analyzed by western blot with anti-P53 antibody. 

Abbr. HO, homozygous; WT, wild type. Note. The hTP53-R273H mice and C57BL/6 mice were i.p. injected with LPS (5mg/kg, 6h).