hPNPLA3-I148M(2)
Nomenclature
C57BL/6JSmo-Pnpla3tm1(hPNPLA3-I148M)Smoc
Cat. NO.
NM-HU-250717
Strain State
Repository Live
Gene Summary
Gene Symbol
Pnpla3
Model Description
Validation Data
Fig.1 Detection of PNPLA3 expression in liver by RT-PCR. Liver RNA was extracted from 7-week-old male wild-type C57BL/6 (WT)(n=1) and homozygous hPNPLA3-I148M(2) knockin mice (HO) (n=2), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mRNA primers. Mouse Pnpla3 mRNA (341 bp) was detectable only in wild-type C57BL/6 mice. Human PNPLA3 mRNA (343 bp) was detectable only in homozygous hPNPLA3-I148M(2) knockin mice but not in wild-type mice.
Fig.2 mRNA sequencing of hPNPLA3-I148M(2) knockin mice.
Abbr. HO, homozygous; WT, wild type.
Fig.3 Detection of PNPLA3 expression in hPNPLA3-I148M(2) mice by WB. The liver tissue lysates were collected from 7-week-old male wild-type C57BL/6 mice and 7-week-old homozygous hPNPLA3-I148M(2) mice, and then analyzed by western blot with anti-PNPLA3 antibody. PNPLA3 was detectable in liver from both WT C57BL/6 and HO hPNPLA3-I148M(2) mice , as the antibody was cross-reactived between human and mouse PNPLA3.
Abbr. HO, homozygous; WT, wild type; PC, positive control, liver tissue lysates.
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