hSNCA(2)

Fig.1 Detection of SNCA expression in WT mice and hSNCA(2) mice by WB. α-synuclein (SNCA) was detectable at a similar level in hippocampus and cortex from both WT C57BL/6 and HE hSNCA(2) mice with an antibody which is cross-reactived between human and mouse SNCA. The hippocampus and cortex lysates were collected from 12-week-old female WT C57BL/6 mice and 7-week-old male heterozygous hSNCA(2) mice, and then analyzed by western blot with an anti-human/mouse SNCA antibody. 

Abbr. HE, heterozygous; WT, wild type. M, marker.

Fig.2 Detection of human SNCA expression in WT mice and hSNCA(2) mice by WB. Human α-synuclein (SNCA) was exclusively detectable in hippocampus and cortex from HE hSNCA(2) mice with a human SNCA-specific antibody. The hippocampus and cortex tissue lysates were collected from 12-week-old female WT C57BL/6 mice and 7-week-old male heterozygous hSNCA(2) mice, and then analyzed by western blot with an anti-human SNCA antibody. 

Abbr. HE, heterozygous; WT, wild type; M, marker.

Fig.3 Detection of SNCA expression in heart, pancreas, small intestine and frontal lobe by RT-PCR. Mouse Snca mRNA (168 bp) was detectable only in wild-type C57BL/6 mice. Human SNCA mRNA (120 bp) was detectable only in homozygous hSNCA(2) knockin mice but not in wild-type mice. Heart, pancreas, small intestine and frontal lobe RNA was extracted from 7-week-old female wild-type C57BL/6 mice (n=2) and homozygous hSNCA(2) knockin mice (n=2), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mRNA primers. 

Abbr. M, marker; HO, homozygous; WT, wild type.