hIL12B/hIL23A/hTNFa-Tg
Nomenclature
C57BL/6JSmo-Tgtn(hTNFa promoter-hTNFa-pA)Il23atm3(hIL23A)Il12btm3(hIL12B)Smoc
Cat. NO.
NM-XA-252108
Strain State
Repository Live
Model Description
Validation Data
Table.1 Blood biochemistry examination in 6-week-old HO hTNFa-Tg/hIL23A/hIL12B mice (n=3 /group).
Table.2 Blood routine examination in 6-week-old HO hTNFa-Tg/hIL23A/hIL12B mice (n=2/group).
Fig.1 Frequency of leukocyte subpopulations in spleen by flow cytometry. Splenocytes were isolated from WT C57BL/6 (WT) mice and HO hTNFa-Tg/hIL23A/hIL12B (HO) mice (male, 6-week-old, n=3). Flow cytometry analysis of frequencies of lymphocyte and myeloid subpopulations in the spleen. Percentages of T cells, B cells, and NK cells in the spleen of HO hTNFa-Tg/hIL23A/hIL12B mice were similar to those in C57BL/6 mice. However, there is a trend of higher frequency of CD11b+myeloid cells, DCs, neutrophils, monocytes, and macrophages in the spleen of humanized mice than in WT C57BL/6 mice, which might be related to the overexpression of humanized TNFα in the mice. Values are expressed as mean ± SEM.
Fig.2 Frequency of leukocyte subpopulations in lymph notes by flow cytometry. Lymph notes cells were isolated from WT C57BL/6 (WT) mice and hTNFa-Tg/hIL23A/hIL12B (HO) mice (male, 6-week-old, n=3). Flow cytometry analysis of frequencies of lymphocyte and myeloid subpopulations in the lymph notes. Percentages of T cells, B cells, NK cells, CD11b+myeloid cells, DCs, neutrophils, monocytes, and macrophages in lymph notes of HO hTNFa-Tg/hIL23A/hIL12B mice were similar to those in C57BL/6 mice, demonstrating that humanization of these genes does not change the frequency or distribution of these cell types in lymph notes. Values are expressed as mean ± SEM.
Fig.3 Human TNF-α levels in serum of humanized hTNFa-Tg/hIL23A/hIL12B mice following LPS stimulation. Wild-type C57BL/6 mice and humanized hTNFa-Tg/hIL23A/hIL12B mice(male, 6 weeks old) were challenged with a single intraperitoneal injection of LPS. Serum concentrations of human TNF-α were measured by ELISA 2 hours post-injection. Human TNF-α was highly expressed in homozygous humanized hTNFa-Tg/hIL23A/hIL12B mice. Data are presented as mean ± SEM from three male mice per group.
You may also like
Shanghai Model Organisms Center Inc has licensed CRISPR-Cas9 technology from Broad Institute
On Dec 16, 2018, Broad Institute and Shanghai Model Organisms Center Inc (SMOC) has entered into a non-exclusive license agreement under which Broad has granted SMOC worldwide rights to commercialize a service platform for genetically modified mouse models under Broad's intellectual property.
Learn moreCustomizing Mouse Models: Delivering in 100 Days to Speed Up Your Research!
At GenoBioTX, we understand that the lengthy wait times for gene-modified mouse models can hinder your research progress. Traditional methods often require 6-9 months, leading to delays and increased costs. That’s why we’re thrilled to introduce our innovative service designed to streamline this process and deliver results faster.
Learn moreMilestones
Our vision is to provide researchers all over the world with comprehensive, convenient and professional animal model services to facilitate a simplified and highly-efficient approach towards uncovering the mysteries of life.
Learn more
