hPLG

Fig.1 Detection of PLG expression in liver by RT-PCR. Mouse Plg mRNA (128 bp) was detectable both in wild-type C57BL/6 mice and heterozygous PLG knockin mice. Human PLG mRNA (160 bp) was detectable only in heterozygous PLG knockin mice but not in wild-type mice. Liver RNA was extracted from C57BL/6 (6-8-week-old,  male, WT, n=3) and hPLG knockin mice (11-week-old, male, HE, n=3), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mRNA primers. 

Abbr. M, marker; HE, heterozygous; WT, wild type.

Fig.2 Detection of human PLG expression in serum of WT C57BL/6 mice and HE hPLG mice by ELISA. Human PLG was exclusively detectable in heterozygous hPLG knockin mice. Serum were collected from WT C57BL/6 mice (6-8 weeks old, female, n=3) and HE hPLG mice (11 weeks old, male, n=3), and analyzed by ELISA with anti-hPLG ELISA kit. 

Abbr. HE, heterozygous; WT, wild type; N.D. not detected.