hFCGR1A/hFCGR2B
Nomenclature
C57BL/6JSmo-Fcgr1tm3(hFCGR1A)Fcgr2btm3(hFCGR2B)Smoc
Cat. NO.
NM-XA-253155
Strain State
Repository Live
Model Description
Validation Data
Fig.1 Detection of FCGR1A expression in subcutaneous fat pad, bladder and liver by RT-PCR. Subcutaneous fat pad, bladder and liver RNA was extracted from 8-week-old male WT C57BL/6 (WT)(n=2) and homozygous hFCGR1A/hFCGR2B mice (HO/WT) (n=2), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mRNA primers.
Abbr. M, marker; HO, homozygous; WT, wild type.
Fig.2 Detection of FCGR2B expression in liver, brain, spleen and bone marrow by RT-PCR. Liver, brain, spleen and bone marrow RNA was extracted from 12-week-old male wild-type C57BL/6 (WT)(n=2) and heterozygous hFCGR1A/hFCGR2B mice (HE/HE) (n=2), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mRNA primers.
Abbr. M, marker; HE, heterozygous; WT, wild type.
Fig.3 Detection of hFCGR2B and mFCGR2B expression on macrophages,monocytes and B cells in spleen of WT C57BL/6 mice and WT/HO hFCGR1A/hFCGR2B mice by FACS.
Leukocytes were collected from wild-type C57BL/6 mice (female, 8 weeks old) and WT/HO hFCGR1A/hFCGR2B humanized mice (female, 9 weeks old), and analyzed by flow cytometry with anti-mCD32 and anti-hCD32.
Abbr. HO, homozygous; WT, wild type.
Fig.4 Detection of hFCGR2B and mFCGR2B expression on macrophages,monocytes and B cells in blood of WT C57BL/6 mice and WT/HO hFCGR1A/hFCGR2B mice by FACS.
Leukocytes were collected from wild-type C57BL/6 mice (female, 8 weeks old) and WT/HO hFCGR1A/hFCGR2B humanized mice (female, 9 weeks old), and analyzed by flow cytometry with anti-mCD32 and anti-hCD32.
Abbr. HO, homozygous; WT, wild type.
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