huPBMC-(MHCII/B2M-KO NMG)

Nomenclature

huPBMC-NOD.Cg-PrkdcscidIl2rgem1H2-Ab1em1H2-Aaem1H2-Eb1em1H2-Eb2em1H2-Ea-ps em1B2mem1Smoc

Cat. NO.

NM-HU-263012

Strain State

Repository Live

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Model Description

MHCII and B2m genes were knocked out on NMG mice. An immune reconstitution model was constructed by transplanting fresh human peripheral blood mononuclear cells (PBMCs) into MHCII/B2M-KO(NMG) mice. This model exhibits a low degree of graft-versus-host disease (GvHD).

Validation Data

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Fig 1. Body weight growth curve, survival rate and GvHD scores of huPBMC-MHC2/B2m-dKO(NMG) mice. 

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Fig 2. Flow cytometry analysis of peripheral blood lymphocyte subpopulations in the huPBMC-MHC2/B2m-dKO(NMG) mouse model. Human PBMCs (1E7) were injected intravenously (female, 6-8 weeks old, n=8). Blood from mice at different time points after implantation of human PBMCs was taken for flow cytometry analysis.

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Fig 3. Efficacy evaluation of BiTE in huPBMC-MHC2/B2m-dKO(NMG) model of small cell lung cancer.

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Fig 4. Efficacy evaluation of BiTE in huPBMC-MHCII/B2M-dKO(NMG) model of lymphoblastic leukemia.

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Fig 5. Efficacy evaluation of anti-PD1 Ab in huPBMC-MHCII/B2M-dKO(NMG) model of malignant melanoma.

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Fig 6. Efficacy evaluation of anti-CD3 Ab in huPBMC-MHCII/B2M-dKO(NMG) model of lymphoblastic leukemia.

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Fig 7. Efficacy evaluation in huPBMC-MHCII/B2M-dKO(NMG) model of liver cancer.

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Fig8. Efficacy evaluation of in vivo CAR-T cell therapy in huPBMC-MHCII/B2M-dKO (NMG) model of lymphoblastic leukemia


For strain background verification data, refer to MHCII/B2m-KO(NMG) NM-NSG-014. Click the link below for more details:

MHCII/B2m-KO(NMG)-SMOC


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