hRBP4

Fig.1 Detection of RBP4 expression in liver by RT-PCR. 

Wild type: only one band at 213 bp with primers F1/R1(mRbp4);

Homozygous:  only one band at 233 bp with primers F2/R2(hRBP4). 

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

Fig.2 Detection of humanized RBP4 mRNA expression by qPCR. 

Relative expression was reported as the percentage to hRBP4 mRNA levels in liver, eyes or kidney of 7-week-old male or 5-week-old female hRBP4 knockin mice, respectively. 

Abbr. HO, homozygous; WT, wild type.

Fig.3 Detection of human RBP4 expression in the kidney of HO hRBP4 mice by WB. 

The human RBP4 expression detected using a cross-reactive antibody that recognizes both mouse and human RBP4 was quantified by measuring the mean intensity.

Abbr. M, marker; WT, wild type; HO, Homozygous.

Fig.4 Detection of human RBP4 expression in the liver of hRBP4 knockin mice by WB. 

The human RBP4 expression was quantified by measuring the mean intensity.

Abbr. M, marker; WT, wild type; HO, Homozygous.

Fig.5 Detection of human RBP4 expression in the eye of hRBP4 knockin mice by WB. 

The human RBP4 expression was quantified by measuring the mean intensity.

Abbr. M, marker; WT, wild type; HO, Homozygous.

Fig.6 Detection of human RBP4(A) and mouse RBP4(B) expression in plasma of WT C57BL/6 mice and HO hRBP4 mice by ELISA. Human RBP4 was exclusively detectable in HO hRBP4 mice. Mouse RBP4 was exclusively detectable in WT C57BL/6 mice. Plasma were collected from wild-type C57BL/6 mice (n=3, 6-7 weeks old) and HO hRBP4 mice (n=3, male, 8 weeks old), and analyzed by ELISA. 

Abbr.  HO, homozygous; WT, wild type; N.D., not detected.