hCA9
Nomenclature
C57BL/6JSmo-Car9tm1(hCA9)Smoc
Cat. NO.
NM-HU-234371
Strain State
Repository Live
Gene Summary
Gene Symbol
Car9
Model Description
Validation Data
Fig.1 Detection of CA9 expression in stomach by RT-PCR.
Wild type: only one band at 144 bp with primers F1/R1(mCar9);
Homozygous: only one band at 186 bp with primers F2/R2(hCA9).
Abbr. M, DNA marker; HO, homozygous; WT, wild type.
Fig.2 Detection of CA9 expression in hCA9 mice by WB.
The stomach lysates were collected from 15-week-old male wild-type C57BL/6 mice and 14-week-old male homozygous hCA9 mice, and then analyzed by western blot with anti-CA9 antibody.
Abbr. HO, homozygous; WT, wild type; M, marker.
Fig.3 Detection of human CA9(A) and mouse CA9(B) expression in plasma of WT C57BL/6 mice and HO hCA9 mice by ELISA. Plasma were collected from wild-type C57BL/6 mice (n=2, male, 15 weeks old) and homozygous hCA9 mice (n=2, male, 14 weeks old), and analyzed by ELISA with anti-hCA9 ELISA kit and anti-mCA9 ELISA kit.
Abbr. HO, homozygous; WT, wild type; N.D. not detected.
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