hGHRL

Fig.1 Detection of GHRL expression in stomach by RT-PCR. Mouse Ghrl mRNA (108 bp) was detectable only in wild-type C57BL/6 mice. Human GHRL mRNA (123 bp) was detectable only in homozygous hGHRL knockin mice but not in wild-type mice. Stomach RNA was extracted from 10-week-old female wild-type C57BL/6 (WT, n=3) and homozygous hGHRL knockin mice (HO, n=3), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mRNA primers. 

Abbr. M, marker; HO, homozygous; WT, wild type.

Fig.2 Detection of human Obestatin expression in serum of WT C57BL/6 mice and HO hGHRL mice by ELISA. Obestatin was detectable in serum from both WT C57BL/6 and homozygous hGHRL knockin mice, as the ELISA kit was cross-reactived between human and mouse Obestatin. Serum were collected from wild-type C57BL/6 mice (n=3, female, 10 weeks old) and HO hGHRL knockin mice (n=3, female, 10 weeks old), and analyzed by ELISA. 

Abbr.  HO, homozygous; WT, wild type.

Fig.3 Detection of human Ghrelin expression in serum of WT C57BL/6 mice and HO hGHRL mice by ELISA. Ghrelin was detectable in serum from both WT C57BL/6 and homozygous hGHRL mice, as the ELISA kit was cross-reactived between human and mouse Ghrelin. Serum were collected from wild-type C57BL/6 mice (n=2, female, 10 weeks old) and HO hGHRL knockin mice (n=2, female, 10 weeks old), and analyzed by ELISA. 

Abbr. HO, homozygous; WT, wild type.

Fig.4 Body weight of WT C57BL/6 mice (6 females, 7.7 weeks old; 12 males, 6.7-7.7 weeks old) and HO hGHRL mice (5 females, 7 weeks old; 11 males, 6.3-7 weeks old). The body weight of homozygous hGHRL mice was slightly higher than WT mice. 

Abbr.  HO, homozygous; WT, wild type.