hCXCR4(2)
Nomenclature
C57BL/6JSmo-Cxcr4tm1(hCXCR4)Smoc
Cat. NO.
NM-HU-251443
Strain State
Repository Live
Gene Summary
Gene Symbol
Cxcr4
Model Description
Validation Data
Fig.1 Detection of CXCR4 expression in thymus by RT-PCR. Mouse Cxcr4 mRNA (248 bp) was detectable only in wild-type C57BL/6 mice. Human CXCR4 mRNA (319 bp) was detectable only in homozygous hCXCR4(2) knockin mice but not in wild-type mice. Thymus RNA was extracted from 6-week-old female wild-type C57BL/6 (WT)(n=2) and homozygous hCXCR4(2) knockin mice (HO) (n=2), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mRNA primers.
Fig.2 Detection of CXCR4 expression on T, B cells and CD11b+ cells in spleen of WT C57BL/6 mice and HO hCXCR4(2) mice by FACS. hCXCR4 was exclusively detectable in hCXCR4(2) humanized mice but not in wild-type mice. hCXCR4 was exclusively detectable on T, B cells and CD11b+ cells in hCXCR4(2) humanized mice, whereas mCXCR4 was exclusively detectable on T, B cells and CD11b+ cells in wild-type C57BL/6 mice. Leukocytes were collected from spleen of wild-type C57BL/6 mice (female, 5 weeks old) and homozygous hCXCR4(2) humanized mice (female, 6 weeks old), and analyzed by flow cytometry with anti-mCXCR4 and anti-hCXCR4.
Abbr. HO, homozygous; WT, wild type.
Fig.3 Detection of CXCR4 expression on T, B cells and CD11b+ cells in BM of WT C57BL/6 mice and HO hCXCR4(2) mice by FACS. hCXCR4 was exclusively detectable in hCXCR4(2) humanized mice but not in wild-type mice. hCXCR4 was exclusively detectable on T, B cells and CD11b+ cells in hCXCR4(2) humanized mice, whereas mCXCR4 was exclusively detectable on T, B cells and CD11b+ cells in wild-type C57BL/6 mice. Leukocytes were collected from BM of wild-type C57BL/6 mice (female, 5 weeks old) and homozygous hCXCR4(2) humanized mice (female, 6 weeks old), and analyzed by flow cytometry with anti-mCXCR4 and anti-hCXCR4.
Abbr. HO, homozygous; WT, wild type.
Fig.4 Detection of ulocuplumab+ on T, B cells and CD11b+ cells in BM of WT C57BL/6 mice and HO hCXCR4(2) mice by FACS. Ulocuplumab+ cells was exclusively detectable in hCXCR4(2) humanized mice but not in wild-type mice. Leukocytes were collected from BM of wild-type C57BL/6 mice (female, 5 weeks old) and homozygous hCXCR4(2) humanized mice (female, 6 weeks old), and analyzed by flow cytometry with ulocuplumab and anti-human IgG.
Abbr. HO, homozygous; WT, wild type.
Note. The positive signals observed in wild-type mice were due to non-specific staining, with a positive rate comparable to that of the FMO (fluorescence minus one) control.
You may also like
Shanghai Model Organisms Center Inc has licensed CRISPR-Cas9 technology from Broad Institute
On Dec 16, 2018, Broad Institute and Shanghai Model Organisms Center Inc (SMOC) has entered into a non-exclusive license agreement under which Broad has granted SMOC worldwide rights to commercialize a service platform for genetically modified mouse models under Broad's intellectual property.
Learn moreWorkshop：Progress and Advances in Preclinical immuno-Oncology Research
SMOC’s Annual Progress and Advances in Preclinical immuno-Oncology Research: The workshop is designed as a forum for ideas and opinions exchange on how to decrease the rate of clinical failures in oncology and immuno-oncology.
Learn moreCustomizing Mouse Models: Delivering in 100 Days to Speed Up Your Research!
At GenoBioTX, we understand that the lengthy wait times for gene-modified mouse models can hinder your research progress. Traditional methods often require 6-9 months, leading to delays and increased costs. That’s why we’re thrilled to introduce our innovative service designed to streamline this process and deliver results faster.
Learn more
