hGRB14(2)

Fig.1 Detection of GRB14 expression in heart and liver by RT-PCR. Mouse Grb14 mRNA (225 bp) was detectable both in wild-type C57BL/6 mice and heterozygous hGRB14(2) knock-in mice. Human GRB14 mRNA (442 bp/872 bp/869 bp/396 bp/346 bp) was detectable only in heterozygous hGRB14(2) knock-in mice but not in wild-type mice. Heart and liver RNA was extracted from 8-week-old male wild-type C57BL/6 (WT)(n=2) and heterozygous hGRB14(2) knock-in mice (HE) (n=2), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mRNA primers. 

Abbr. M, marker; HE, heterozygous; WT, wild type.

Fig.2 Detection of GRB14 expression in hGRB14(2) mice by WB. GRB14 was detectable in the liver from both WT C57BL/6 and HE hGRB14(2) mice, as the antibody was cross-reactived between human and mouse GRB14.  The liver lysates were collected from 8-week-old male wild-type C57BL/6 mice and 8-week-old male heterozygous hGRB14(2) mice, and then analyzed by western blot with anti-GRB14 antibody. 

Abbr. HE, heterozygous; WT, wild type; PC, positive control, PC1, HepG2 cells, PC2, HT-1080 cells; M, marker.

Fig.3 Detection of GRB14 expression in hGRB14(2) mice by WB. GRB14 was detectable in heart and small intestine tissues from both WT C57BL/6 and HE hGRB14(2) mice, as the antibody was cross-reactived between human and mouse GRB14. The heart and small intestine lysates were collected from 8-week-old male wild-type C57BL/6 mice and 8-week-old male heterozygous hGRB14(2) mice, and then analyzed by western blot with anti-GRB14 antibody. 

Abbr. HE, heterozygous; WT, wild type; PC, positive control, PC1, HepG2 cells, PC2, HT-1080 cells; M, marker.