hALKBH5(2)
Nomenclature
C57BL/6JSmo-Alkbh5tm1(hALKBH5)Smoc
Cat. NO.
NM-HU-251634
Strain State
Repository Live
Gene Summary
Gene Symbol
Alkbh5
Model Description
Validation Data
Fig.1 Detection of ALKBH5 expression in kidney by RT-PCR. Kidney RNA was extracted from 7-week-old male wild-type C57BL/6 (WT) and homozygous hALKBH5(2) knockin mice (HO) (n=3), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mRNA primers. Mouse Alkbh5 mRNA (621 bp) was detectable only in wild-type C57BL/6 mice. Human ALKBH5 mRNA (500 bp) was detectable only in homozygous hALKBH5(2) knockin mice but not in wild-type mice.
Fig.2 Detection of ALKBH5 expression in hALKBH5(2) mice by WB. The lysates of brain, skeletal muscle and eWAT tissues were collected from 7-week-old male WT C57BL/6 mice and homozygous hALKBH5(2) mice, and then analyzed by western blot with anti-ALKBH5 antibody. ALKBH5 was detectable in brain, skeletal muscle and eWAT tissues from both WT C57BL/6 and HO hALKBH5(2) mice, as the antibody was cross-reactived between human and mouse ALKBH5.
Abbr. HO, homozygous; WT, wild type; PC, positive control, Hela cells; M, marker; eWAT, epididymal white adipose tissue.
You may also like
Shanghai Model Organisms Center Inc has licensed CRISPR-Cas9 technology from Broad Institute
On Dec 16, 2018, Broad Institute and Shanghai Model Organisms Center Inc (SMOC) has entered into a non-exclusive license agreement under which Broad has granted SMOC worldwide rights to commercialize a service platform for genetically modified mouse models under Broad's intellectual property.
Learn moreWorkshop：Progress and Advances in Preclinical immuno-Oncology Research
SMOC’s Annual Progress and Advances in Preclinical immuno-Oncology Research: The workshop is designed as a forum for ideas and opinions exchange on how to decrease the rate of clinical failures in oncology and immuno-oncology.
Learn moreCustomizing Mouse Models: Delivering in 100 Days to Speed Up Your Research!
At GenoBioTX, we understand that the lengthy wait times for gene-modified mouse models can hinder your research progress. Traditional methods often require 6-9 months, leading to delays and increased costs. That’s why we’re thrilled to introduce our innovative service designed to streamline this process and deliver results faster.
Learn more
