hFAP(2)

Fig.1 Detection of hFAP expression on CD45- cells in tumor of WT C57BL/6 mice and HO hFAP(2) mice by FACS.

Cells were collected from tumor of wild-type C57BL/6 mice (female, 15 weeks old) and homozygous hFAP(2) humanized mice (female, 15 weeks old), and analyzed by flow cytometry with anti-hFAP. 

Abbr. HO, homozygous; WT, wild type.

Fig.2 Detection of human FAP(A) and mouse FAP(B) expression in serum of WT C57BL/6 mice and HO hFAP(2) mice by ELISA.  

Serum were collected from wild-type C57BL/6 mice (n=3, female, 12 weeks old) and HO hFAP(2) mice (n=3, female, 12 weeks old), and analyzed by ELISA with anti-hFAP ELISA kit (Abcam, ab256404) and anti-mFAP ELISA kit. 

Abbr.  HO, homozygous; WT, wild type; N.D. not dected.

Fig.3 Tumor growth and body weight in the Panc02 subcutaneous model. Pan02 cells (1×106) were subcutaneously implanted into HO hFAP(2) KI mice (female and male, n=10, 9 weeks old) and WT C57BL/6 (female and male, n=10, 6-8 weeks old). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Tumor volume was calculated using the formula: V = (L × W²) / 2, where L is the longest diameter and W is the shortest diameter perpendicular to L.

Abbr. HO, homozygous; WT, wild type.

Fig.4 Frequency of lymphocyte and myeloid subpopulations in the tumor of WT C57BL/6 mice and HO hFAP(2) KI mice. Single cells were isolated from Panc02 subcutaneous tumors in wild-type C57BL/6 mice(n=7) and homozygous hFAP(2) humanized mice (n=5), and analyzed by flow cytometry. Bars represente as mean ± SEM.

Abbr. HO, homozygous; WT, wild type.