R26-CAG-MYOC mutant-3xFlag-linker-3xHA
Nomenclature
C57BL/6JSmo-Gt(ROSA)26Sorem1(CAG-MYOC mutant-3xFlag-linker-3xHA)Smoc
Cat. NO.
NM-KI-240582
Strain State
Sperm cryopreservation
Model Description
Validation Data
Fig.1 Detection of MYOC expression in bladder, liver and cortex by RT-PCR. Human MYOC mRNA (341 bp) was detectable only in homozygous R26-CAG-MYOC mutant-3xFlag-linker-3xHA mice but not in wild-type miceBladder, liver and cortex RNA was extracted from 6-week-old male and female wild-type C57BL/6 (WT)(n=2) and homozygous R26-CAG-MYOC mutant-3xFlag-linker-3xHA mice (HO) (n=2), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mRNA primers.
Abbr. M, marker; HO, homozygous; WT, wild type.
Fig.2 mRNA sequencing of R26-CAG-MYOCmutant-3xFlag-linker-3xHA mice. Sequencing data of RT-PCR products confirmed the presence of target mutations in R26-CAG-MYOC mutant-3xFlag-linker-3xHA mice.
Abbr. HO, homozygous; WT, wild type.
Fig.3 Detection of Flag expression in R26-CAG-MYOCmutant-3xFlag-linker-3xHA mice by WB. Flag was exclusively detectable in liver and eyeball from HO R26-CAG-MYOC mutant-3xFlag-linker-3xHA mice.The liver and eyeball tissue lysates were collected from 6-week-old wild-type C57BL/6 mice and 6-week-old homozygous R26-CAG-MYOCmutant-3xFlag-linker-3xHA mice, and then analyzed by western blot with anti-Flag antibody .
Abbr. HO, homozygous; WT, wild type; M, marker.
Fig.4 Detection of MYOC expression in R26-CAG-MYOCmutant-3xFlag-linker-3xHA mice by IF. The eyeball were collected from 6-week-old wild-type C57BL/6 mice and 6-week-old homozygous R26-CAG-MYOC mutant-3xFlag-linker-3xHA mice, and then analyzed by IF with anti-Flag antibody .
Abbr. HO, homozygous; WT, wild type; M, marker.
You may also like
Shanghai Model Organisms Center Inc has licensed CRISPR-Cas9 technology from Broad Institute
On Dec 16, 2018, Broad Institute and Shanghai Model Organisms Center Inc (SMOC) has entered into a non-exclusive license agreement under which Broad has granted SMOC worldwide rights to commercialize a service platform for genetically modified mouse models under Broad's intellectual property.
Learn moreWorkshop：Progress and Advances in Preclinical immuno-Oncology Research
SMOC’s Annual Progress and Advances in Preclinical immuno-Oncology Research: The workshop is designed as a forum for ideas and opinions exchange on how to decrease the rate of clinical failures in oncology and immuno-oncology.
Learn moreCustomizing Mouse Models: Delivering in 100 Days to Speed Up Your Research!
At GenoBioTX, we understand that the lengthy wait times for gene-modified mouse models can hinder your research progress. Traditional methods often require 6-9 months, leading to delays and increased costs. That’s why we’re thrilled to introduce our innovative service designed to streamline this process and deliver results faster.
Learn more
