hCD3EDG/hCD19/hCD28

Nomenclature

C57BL/6JSmo-Cd28tm2(hCD28)Cd3etm1(hCD3E)Cd3dtm1(hCD3D)Cd3gtm1(hCD3G)Cd19tm1(hCD19)Smoc

Cat. NO.

NM-XA-240981

Strain State

Repository Live

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Model Description

The hCD3EDG/hCD19(NM-HU-220109) was crossed with hCD28(NM-HU-190011) to generate hCD28/hCD3EDG/hCD19 mice.

Validation Data

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Fig.1 CD3 specific expression analysis in WT C57BL/6 and HO/HO/HO hCD3EDG/hCD28/hCD19 mice in peripheral blood, lymph node and spleen.

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Fig.2 CD19 specific expression analysis in WT C57BL/6 and HO/HO/HO hCD3EDG/hCD28/hCD19 mice in peripheral blood, lymph node and spleen.

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Fig.3 CD28 specific expression analysis in WT C57BL/6 and HO/HO/HO hCD3EDG/hCD28/hCD19 mice in peripheral blood, lymph node and spleen.

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Fig.4 Frequency of leukocyte subpopulations in WT C57BL/6 and HO/HO/HO hCD3EDG/hCD28/hCD19 mice by flow cytometry.

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Fig.5 Frequency of leukocyte subpopulations in WT C57BL/6 and HO/HO/HO hCD3EDG/hCD28/hCD19 mice in (A)  lymph node, (B) peripheral blood(PB) and (C) spleen by flow cytometry(female, 5-6-week-old, n=3).

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Fig.6 Organ index of WT C57BL/6 and HO/HO/HO hCD3EDG/hCD28/hCD19 mice (female, 5-6-week-old, n=3).

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Table 1. Blood routine examination of hCD3EDG/hCD19/hCD28 knockin mice. Values are expressed as Mean±SEM.

Abbr. HO, homozygous; WT, wild type.

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Table 2. Biochemical test of serum from hCD3EDG/hCD19/hCD28 knockin mice. Values are shown as Mean±SEM.

Abbr. HO, homozygous; WT, wild type.

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Fig.7 Detection immunoglobulin levels in serum by ELISA. 

Serum was collected from wild-type C57BL/6 mice (WT) and homozygous hCD3EDG/hCD19/hCD28 mice (HO/HO/HO) (n=8 in each group, 7-8 weeks old, Mean±SEM). 

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Fig.8 Detection C3c and C4 levels in serum by ELISA. 

Serum was collected from wild-type C57BL/6 mice (WT) and homozygous hCD3EDG/hCD19/hCD28 mice (HO/HO/HO) (n=3-8 for C3c detection and n=8 for C4 detection, 7-8 weeks old, Mean±SEM).

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