hCD5(BALB/c)
Nomenclature
BALB/cJSmo-Cd5tm1(hCD5)Smoc
Cat. NO.
NM-HU-241178
Strain State
Repository Live
Gene Summary
Gene Symbol
Cd5
Model Description
Validation Data
Fig.1 Detection of CD5 expression in thymus by RT-PCR. Mouse Cd5 mRNA (162 bp) was detectable only in wild-type BALB/c mice. Human CD5 mRNA (249 bp) was detectable only in homozygous hCD5 (BALB/c) knockin mice but not in wild-type mice. Thymus RNA was extracted from 7-week-old female wild-type BALB/c (WT)(n=3) and homozygous hCD5 (BALB/c) knockin mice (HO) (n=3), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mRNA primers.
Abbr. M, marker; HO, homozygous; WT, wild type.
Fig.2 Detection of CD5 expression on T cells and B cells in spleen of WT (BALB/c) mice and HO hCD5(BALB/c) mice by FACS. hCD5 was exclusively detectable in hCD5(BALB/c) humanized mice but not in wild-type (BALB/c) mice. hCD5 was exclusively detectable on T cells and B cells in hCD5(BALB/c) humanized mice, whereas mCD5 was exclusively detectable on T cells and B cells in wild-type BALB/c mice. Leukocytes were collected from spleen of wild-type BALB/c mice (female, 6 weeks old) and homozygous hCD5(BALB/c) humanized mice (female, 6 weeks old), and analyzed by flow cytometry with anti-mCD5 and anti-hCD5.
Abbr. HO, homozygous; WT, wild type.
Fig.3 Detection of CD5 expression on T cells and B cells in blood of WT (BALB/c) mice and HO hCD5(BALB/c) mice by FACS. hCD5 was exclusively detectable in hCD5(BALB/c) humanized mice but not in wild-type (BALB/c) mice. hCD5 was exclusively detectable on T cells and B cells in hCD5(BALB/c) humanized mice, whereas mCD5 was exclusively detectable on T cells and B cells in wild-type BALB/c mice. Leukocytes were collected from blood of wild-type BALB/c mice (female, 6 weeks old) and homozygous hCD5(BALB/c) humanized mice (female, 6 weeks old), and analyzed by flow cytometry with anti-mCD5 and anti-hCD5.
Abbr. HO, homozygous; WT, wild type.
Fig.4 Frequency of leukocyte and myeloid subpopulations in the blood by flow cytometry. hCD5(BALB/c) knockin mice showed a normal immune phenotype in blood. Blood was collected from WT BALB/c mice and HO hCD5(BALB/c) mice (6-week-old, n=3). Flow cytometry analysis of frequencies of (A) lymphocyte and (B) myeloid subpopulations in the blood. Bar indicated expressed as mean ± SEM.
Abbr. HO, homozygous; WT, wild type.
Fig.5 Frequency of leukocyte and myeloid subpopulations in the spleen by flow cytometry. hCD5(BALB/c) knockin mice showed a normal immune phenotype in spleen. Spleen was collected from WT BALB/c mice and HO hCD5(BALB/c) mice (6-week-old, n=3). Flow cytometry analysis of frequencies of (A) lymphocyte and (B) myeloid subpopulations in the spleen. Bar indicated expressed as mean ± SEM.
Abbr. HO, homozygous; WT, wild type.
You may also like
Shanghai Model Organisms Center Inc has licensed CRISPR-Cas9 technology from Broad Institute
On Dec 16, 2018, Broad Institute and Shanghai Model Organisms Center Inc (SMOC) has entered into a non-exclusive license agreement under which Broad has granted SMOC worldwide rights to commercialize a service platform for genetically modified mouse models under Broad's intellectual property.
Learn moreCustomizing Mouse Models: Delivering in 100 Days to Speed Up Your Research!
At GenoBioTX, we understand that the lengthy wait times for gene-modified mouse models can hinder your research progress. Traditional methods often require 6-9 months, leading to delays and increased costs. That’s why we’re thrilled to introduce our innovative service designed to streamline this process and deliver results faster.
Learn moreMilestones
Our vision is to provide researchers all over the world with comprehensive, convenient and professional animal model services to facilitate a simplified and highly-efficient approach towards uncovering the mysteries of life.
Learn more
