hCACNG1

Fig.1 Detection of CACNG1 expression in skeletal muscle by RT-PCR. Mouse Cacng1 mRNA (238 bp) was detectable only in wild-type C57BL/6 mice. Human CACNG1 mRNA (256 bp) was detectable only in homozygous hCACNG1 knockin mice but not in wild-type mice. Skeletal muscle RNA was extracted from 6-week-old male wild-type C57BL/6 (WT) (n=2) and 5-week-old male homozygous hCACNG1 knockin mice (HO) (n=2), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mRNA primers. 

Abbr. M, marker; HO, homozygous; WT, wild type.

Fig.2 Detection of CACNG1 expression in hCACNG1 mice by WB. CACNG1 was detectable in heart, skeletal muscle and tongue tissues from both WT C57BL/6 and HO hCACNG1 mice, as the antibody was cross-reactived between human and mouse CACNG1. The heart, skeletal muscle and tongue tissue lysates were collected from 6-week-old male wild-type C57BL/6 mice and 6-week-old male homozygous hCACNG1 mice, and then analyzed by western blot. 

Abbr. HO, homozygous; WT, wild type.