hCCL20

Fig.1 Detection of CCL20 expression in small intestine and duodenum by RT-PCR. Mouse Ccl20 mRNA (174 bp) was detectable only in wild-type C57BL/6 mice. Human CCL20 mRNA (138 bp) was detectable only in homozygous hCCL20 knockin mice but not in wild-type mice. Small intestine and duodenum RNA was extracted from 6-week-old female wild-type C57BL/6 (WT)(n=2) and homozygous hCCL20 knockin mice (HO) (n=2), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mRNA primers. 

Abbr. M, marker; HO, homozygous; WT, wild type.

Fig.2 Detection of human CCL20(A) and mouse CCL20(B) expression in serum of WT C57BL/6 mice and HO hCCL20 mice by ELISA.  Human CCL20 was exclusively detectable in homozygous hCCL20 knockin mice. Mouse CCL20 was exclusively detectable in wild-type C57BL/6 mice.  Serum were collected from wild-type C57BL/6 mice (n=2, female, 7 weeks old) and HO hCCL20 mice (n=2, female, 7 weeks old), and analyzed by ELISA with anti-mCCL20 ELISA kit and anti-hCCL20 ELISA kit. 

Abbr.  HO, homozygous; WT, wild type.

Note. hCCL20 and C57BL/6 mice were i.p. injected with LPS(5mg/kg) for 3h.