R26-CAG-LSL-hPNPLA3-I148M-EGFP/Alb-cre-Tg
Nomenclature
C57BL/6JSmo-Gt(ROSA)26Sortm1(SA-Frt-2xpolyA-CAG-Frt-LSL-PNPLA3*I148M-IRES-GFP-wpre-polyA)Tg(Alb-Cre)Smoc
Cat. NO.
NM-XA-242889
Strain State
Repository Live
Model Description
Validation Data
Fig.1 Detection of human PNPLA3 expression in liver by qPCR. Each value in G1 and G2 represents the mRNA level relative to the value in G3(n=3 for G1 and G3, n=1 for G2).
Abbr. WT, wild type.
Fig.2 Detection of human PNPLA3 expression in liver by WB.
Abbr. WT: wild type; HE/+: R26CAG-LSL-hPNPLA3-I148M-EGFP/+; Alb-Cre-Tg. HE/-: R26CAG-LSL-hPNPLA3-I148M-EGFP/+.
Fig.3 The body weight curve of R26-CAG-LSL-hPNPLA3-I148M-EGFP/Alb-cre-Tg and C57BL/6 mice. (n=3-8).
Abbr. WT, wild type; CD, chow diet; HSD, high-sugar diet.
Fig.4 Blood chemistry and blood lipid profile in serum of R26-CAG-LSL-hPNPLA3-I148M-EGFP/Alb-cre-Tg and C57BL/6 mice after 6 weeks post diet feeding. (n=6-9, male/female).
Abbr. WT, wild type; CD, chow diet; HSD, high-sugar diet.
Fig.5 TG and T-CHO in liver tissues of R26-CAG-LSL-hPNPLA3-I148M-EGFP/Alb-cre-Tg and C57BL/6 mice after 6 weeks post diet feeding. (n=3-4, male/female).
Abbr. WT, wild type; CD, chow diet; HSD, high-sugar diet.
Fig.6 Representative H&E staining images of liver of R26-CAG-LSL-hPNPLA3-I148M-EGFP/Alb-cre-Tg and C57BL/6 mice after 6 weeks post diet feeding. (n=3-4，Each image is from a different mouse).
Abbr. WT, wild type; CD, chow diet; HSD, high-sugar diet.
Fig.7 Representative masson staining images of liver of R26-CAG-LSL-hPNPLA3-I148M-EGFP/Alb-cre-Tg and C57BL/6 mice after 6 weeks post diet feeding. (n=3-4, Each image is from a different mouse).
Abbr. WT, wild type; CD, chow diet; HSD, high-sugar diet.
G1: C57BL/6, CD; G2: C57BL/6, HSD1; G3: R26CAG-LSL-hPNPLA3-I148M-EGFP/+; Alb-Cre-Tg, HSD1; G4: R26CAG-LSL-hPNPLA3-I148M-EGFP/CAG-LSL-hPNPLA3-I148M-EGFP; Alb-Cre-Tg, CD; G5: R26CAG-LSL-hPNPLA3-I148M-EGFP/CAG-LSL-hPNPLA3-I148M-EGFP; Alb-Cre-Tg, HSD1
Fig.8 Representative Oil red O staining images of liver of R26-CAG-LSL-hPNPLA3-I148M-EGFP/Alb-cre-Tg and C57BL/6 mice after 6 weeks post diet feeding. (n=3-4, Each image is from a different mouse).
Abbr. WT, wild type; CD, chow diet; HSD, high-sugar diet.
G1: C57BL/6, CD; G2: C57BL/6, HSD1; G3: R26CAG-LSL-hPNPLA3-I148M-EGFP/+; Alb-Cre-Tg, HSD1; G4: R26CAG-LSL-hPNPLA3-I148M-EGFP/CAG-LSL-hPNPLA3-I148M-EGFP; Alb-Cre-Tg, CD; G5: R26CAG-LSL-hPNPLA3-I148M-EGFP/CAG-LSL-hPNPLA3-I148M-EGFP; Alb-Cre-Tg, HSD1
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